Medical Microbiology and Parasitology
Oxford Nanopore MinION Mk1C
At the Department of Microbiology, we employ the Oxford Nanopore MinION Mk1C for comprehensive whole genome sequencing (WGS). This portable, high-throughput device enables us to decode the complete DNA sequence of microorganisms, providing detailed insights into their genetic makeup.
The MinION Mk1C operates by passing DNA strands through nanopores, generating real-time data on the nucleotide sequence. This technology allows for long-read sequencing, which is crucial for assembling complex genomes and identifying genetic variations with high accuracy.
Using the MinION Mk1C, we can swiftly and effectively analyze microbial genomes, aiding in outbreak investigations, antibiotic resistance studies, and pathogen characterization. Our commitment to using advanced WGS technologies like the MinION Mk1C ensures that we stay at the forefront of microbial research, contributing to improved public health and scientific discovery.
Contact: Prof. Dr. Chan Yean Yean
Biorad CFX96 Touch Real-Time PCR Detection System
At the Department of Microbiology, we employ the Biorad CFX96 qPCR for development of diagnostic assays to detect various pathogens including bacteria, viruses, and fungi. Bio-Rad CFX96 PCR Detection System is a versatile and high-performance instrument designed for precise and reliable quantitative PCR (qPCR) applications, including microbial detection. It features a six-channel detection system (five colors and one FRET channel), enabling the detection of multiple targets in a single reaction. The system combines advanced optical technology with precise thermal control, ensuring sensitive and accurate fluorescence detection without cross-talk. With a thermal gradient capability, it allows for the optimization of reaction conditions in a single run. The CFX96 Touch can operate independently without a computer, with real-time data visualization on the integrated LCD touch screen. Its high sensitivity and multiplexing capabilities make it ideal for microbial detection applications, including pathogen detection, antibiotic resistance studies, and microbial quantification.
Contact: Prof. Dr. Chan Yean Yean
Conventional PCR Thermocycler
A PCR thermocycler is a vital instrument in microbiology laboratories, facilitating the amplification of specific DNA sequences through the Polymerase Chain Reaction (PCR) technique. By precisely controlling temperature cycles, it enables the denaturation of DNA, annealing of primers, and extension of new DNA strands, thereby exponentially increasing the quantity of targeted DNA segments. This capability is crucial for various applications, including pathogen detection, genetic analysis, and diagnostic testing. Modern thermocyclers offer advanced features such as gradient capabilities for optimizing annealing temperatures and real-time monitoring for quantitative PCR (qPCR), enhancing the versatility and accuracy of molecular analyses. In the Department of Microbiology, PCR thermocyclers are indispensable tools that support advancements in microbial diagnostics and treatment strategies.
Contact: Prof. Dr. Chan Yean Yean
Biosafety Cabinet Class II
A Class II Biosafety Cabinet (BSC) is an essential piece of equipment in the Department of Microbiology & Parasitology, providing critical containment and protection during the handling of infectious agents and hazardous biological materials. Designed to safeguard laboratory personnel, the environment, and the samples being worked with, these cabinets feature inward airflow at the front, downward laminar flow over the work surface, and HEPA-filtered exhaust air. This design ensures that any potential contaminants are contained within the cabinet, preventing exposure to the user and the external environment. Incorporating a Class II BSC into laboratory practices enhances biosafety and supports the integrity of microbiological and parasitological research.
Orbital Incubator Shaker
An orbital incubator shaker is a vital instrument in the Department of Microbiology & Parasitology, combining precise temperature control with gentle, uniform agitation to support the growth and maintenance of microbial cultures. Operating within a temperature range of +5°C to 60°C, these devices facilitate optimal conditions for cultivating bacteria, yeast, fungi, and other microorganisms. The orbital motion ensures consistent mixing, promoting aeration and nutrient distribution, which is crucial for applications such as fermentation studies, enzyme production, and antimicrobial testing. Compact and versatile, orbital incubator shakers are equipped with adjustable shaking speeds and interchangeable platforms, accommodating various sample sizes and types. Their ability to maintain stable conditions enhances reproducibility and reliability in experimental outcomes, making them indispensable tools for advanced microbiological and parasitological research.
CO2 Incubator
A CO₂ incubator is a crucial piece of equipment in the Department of Microbiology & Parasitology, providing a controlled environment essential for the cultivation of microorganisms and cells. These incubators precisely regulate parameters such as temperature (typically 37°C), humidity (around 95%), and carbon dioxide concentration (approximately 5%), which are vital for maintaining the physiological conditions required for optimal growth and metabolic activity. By creating an atmosphere that closely mimics natural biological conditions, CO₂ incubators support a wide range of applications, including microbial diagnostics, tissue culture, virology, and antimicrobial research. Their role is indispensable in ensuring the accuracy and reliability of experimental outcomes in microbiological and parasitological studies.
Computer Workstation
The Department of Microbiology & Parasitology is equipped with a state-of-the-art bioinformatics workstation, featuring the Intel® Core™ Ultra 9 Processor 285K, NVIDIA GeForce RTX 5080 GPU, and 64GB DDR5 RAM. The Intel® Core™ Ultra 9 Processor 285K boasts 24 cores (8 performance cores and 16 efficient cores), with a maximum turbo frequency of 5.7 GHz, providing exceptional computational power for complex analyses. Paired with the NVIDIA GeForce RTX 5080 GPU, this system delivers advanced graphics processing capabilities, accelerating tasks such as data visualization and 3D modeling. The 64GB DDR5 RAM ensures smooth multitasking and efficient handling of large datasets, while the high-speed memory interface supports rapid data access. This workstation is designed to meet the demanding requirements of bioinformatics applications, enabling researchers to perform genomic sequencing, phylogenetic analysis, and other computational tasks with enhanced speed and accuracy.