Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Immunofluorescence (IF) using HEp-2 substrate.

Interpretation: Positive IF (nucleus) at titre 1/80 or more. IF patterns suggest ANA specificity.

Clinical significance: ANA is a screening test for suspected connective tissue disease. Positive result, especially high titres indicate a possible connective tissue disease. Negative result in 95% to 99% of cases rule out systemic lupus erythematosus (SLE). ANA is also detected (low titre) in other autoimmune diseases such as chronic active hepatitis (CAH), Sjögren’s syndrome, primary biliary cirrhosis (PBC) and rheumatoid arthritis (RA).

Result: Within 8 working days.

Note: If ANA is positive, confirm with test for anti-dsDNA, anti-Sm and anti-RNP for SLE [see Anti- Extractable Nuclear Antigens (ENA)].

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Enzyme-linked Immunosorbent Assay (ELISA).

Clinical significance: High titre is suggestive of active SLE, however it is negative in 25% of SLE patients.

Result: Within 8 working days.

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Immunofluorescence (IF) using tissue section as substrate.

Interpretation: Positive IF at titre of 1/10 or more.

Clinical significance: High titre of ASMA is detected in patients with autoimmune chronic active hepatitis (CAH) (60-70%), lupoid hepatitis (80%) and other liver diseases including Epstein Barr viral infection.

Result: Within 10 working days.

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Immunofluorescence (IF) using tissue section as substrate.

Interpretation: Positive IF at titre of 1/10 or more.

Clinical significance: High titre of AMA is detected in 90% patients with primary biliary cirrhosis (PBC) but also found in other liver diseases (low titre), syphilis, connective tissue disorders and myocarditis.

Result: Within 10 working days.

Note: Both AMA & ASMA tests are done together.

Extractable Nuclear Antigen Antibodies (dsDNA, Nucleosomes, Histones, SmD1, PCNA, RPP/PO, SS-A/Ro 60kD, SS-A/Ro 52 kD, SS-B/La, CENP-B, Scl-70, U1-snRNP, AMA M2, Jo-1, PM-Scl, Mi-2 & Ku)

Type of specimen: Minimum 5 ml of blood in plain tube

Method/Principle: The test is based on the principle of an enzyme immunoassay. The test strip is composed of a membrane fixed on a plastic support. The intensity of the coloration is directly proportional to the amount of antibody present in the sample.
Interpretation: A sample is positive for a specific antibody if the colour intensity of the corresponding Antigen Dot is higher than the intensity of the Negative Control Dot. A sample is negative for a specific antibody if the colour intensity of the corresponding Antigen Dot is lower or equal than the intensity of the Negative Control Dot.

Clinical significance: Anti-Sm is specific for SLE (30-40%), but negative result does not rule out SLE. Anti-RNP is detected in mixed connective tissue diaseses (MCTD) (95-100%) but also in SLE and scleroderma. Anti-SS-A occurs in SLE (25%), Sjogren syndrome (40-50%), congenital complete heart block, cutaneous lupus and is a serologic marker for neonatal lupus. Patients with anti-SS-B most commonly have Sjogren syndrome (23%) and SLE (5-10%) and Sjogren syndrome sicca complex (60%) and neonatal lupus. Anti-Scl-70 occurs in 70% of patients with progressive systemic sclerosis (PSS) and is a specific marker for PSS. Anti-Jo-1 is detected in patients with polymyositis/dermatosytis. Anti-PM-Scl is found in patients with polymyositis and scleroderma. Anti- CENP-A/B is detected in patients with CREST (60%). Anti-PCNA is another specific marker for SLE but is only detected in 5-10% of the patients. Anti-Ku and anti-Mi-2 antibodies are detected in patients with myositis.

Result: Within 5 working days.

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Immunofluorescence (IF) using a monolayer of cells as substrate.

Interpretation: Positive IF at titer of 1/20 or more.

Clinical significance: cytoplasmic (c-ANCA) is detected in patients with Wegener’s granulomatosis, microscopic polyarteritis. Perinuclear (p-ANCA) may occur in polyarteritis nodosa, SLE, severe vasculitis and rheumatoid vasculitis.

Result: Within 10 working days.

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: ELISA

Interpretation: the absorbance values of the samples are multiplied by the Conversion Factor to obtain the anti-cardiolipin antibody concentration in GPL or MPL units.

Clinical significance: Positive anti-cardiolipin Ab is found in patients with repeated abortions associated with SLE, various venous and arterial thrombotic disorders including cerebral infarction, deep venous thrombosis, thrombocytopenia and pulmonary embolism.

Result: Within 10 working days.

Type of specimen: 20 ml of urine in urine container (preferable early morning urine).

Method/Principle: Immunoblot (strip).

Interpretation: Band formation is compared with the control. 2 bands formation indicate positive result.

Clinical significance: Positive result indicates pregnancy (in pregnancy hCG is increased exponentially for the first 8 weeks, peaks at 10 weeks and start to decline after that until 1/5 of peak level). But very high hCG up to 200,000IU/L suggestive of chrio-epithelioma. hCG can also be detected in other conditions such as persistent trophoblastic diseases, hydatidiform mole, chorioepithelioma of uterus or testis and testicular tumours (monitoring some ovarian and testicular malignancies). False positive UPT can be seen in UTI (urine full of bacteria), proteinuria, hematuria and patient on methadone treatment. False negative UPT maybe found in ectopic pregnancy, toxaemia, foetal death or threatened abortion.

Result: Within 8 working hours.

Type of specimen: Minimum 5 ml of blood in plain tube.

Method/Principle: Latex agglutination

Interpretation: Agglutination of latex particles compared to positive control.

Clinical significance: RF is detected in >80% patients with rheumatoid arthritis (RA). It is also detected in other diseases such as SLE, Sjogren’s syndrome, chronic infections (infective endocarditis & tuberculosis).

Result: Within 8 working hours.

Type of specimen: minimum 5 ml blood in plain bottle.

Method/principle: Patients samples, calibrators and controls are incubated with the allergen. After washing, all these solid phase are incubated with a conjugate. After another series of washing, a flurogenic substrate is incubated with these solid phase and then stop solution is added. The end points are read by fluorometry. The standard curve is generated. Calibration data are fit and control values are interpolated on the standard curve.

Clinical Significance: Assessing the level of allergen specific IgE in a Patients’ serum in conjunction with a clinical evaluation based on patient history. This will help the clinician to confirm a diagnosis of allergy and assist in the treatment of the patient.Result: within 5 working days.

Result: Within 8 working days

Type of specimen: minimum 10 ml of blood in EDTA bottle

Method/Principle: Extraction of genomic DNA from the mononuclear cells followed by  low-resolution  sequence-specific primer amplification (PCR- SSP).Primer pairs are designed to have perfect matches only with a single allele or group of alleles.  The perfectly  matched primer pairs result in the amplification of target sequences while mismatched primer pairs do not result in amplification.

Interpretation: The presence or absence of appropriately sized bands will be assessed and analyzed using worksheet.  The results of molecular typing were converted to the

serologically   stablished  HLA  phenotypic equivalents.

Clinical Significance: Pre-requisite for organ especially bone marraw transplantation in order to find matched donor.   Tissue typing is done for HLA class I (HLA-A, HLA-B, HLA-C) and class II  (HLA-DR, HLA-DQ).

Result:  3 months

Note: An appointment with the Laboratory is essential for this test. Blood sample of donors and recipient need to be send together.   For donors HLA class II wil be typed first, if DR locus is matched, then  proceed for HLA class I

Type of specimen: paediatric - 3 ml of fresh anti-coagulated blood in EDTA container; adult – 5 ml fresh anti-coagulated blood in EDTA container.

Method/Principle: Flow cytometry using monoclonal antibody against various lymphocyte subpopulations.

Interpretation: Percentage of cells with positive staining.

Clinical significance: Lymphocyte immunophenotyping is mainly done to support diagnosis of AIDS/HIV infection (low CD4 population (<200 cells/ul of blood is characteristic of AIDS). Lymphocyte counts are also useful for B & T cells deficiencies.

Result: Within 8 working hours. 

Note: An appointment with the Laboratory is essential for this test (at least one day earlier). All  specimen must arrive at Immunology Laboratory before 12pm and within 4 hours of collection. 

Type of specimen: Minimum 5 ml of blood in plain container.

Method/Principle: FEIA (Fluorescent enzyme immunoassay).

Interpretation: Test is considered positive if the antibody titre was greater than 12 IU/ml.

Clinical significance: Highly specific marker with comparable sensitivity for rheumatoid arthritis compared to RF.

Results: 10 working days.

Type of specimen: Paediatrics: 1 ml of fresh blood in heparinised tube

Method/Principle: Flow cytometry

Interpretation: Percentage of oxidative burst activity of granulocyte (%) and stimulation index (SI)

Clinical significance: Chronic granulomatous diseases and primary immunodeficiency disorders. 

Results: Within 1 working day.

Note: An appointment with the Laboratory is essential for this test (at least one day earlier). All specimen must arrive at Immunology Laboratory before 12pm and within 4 hours of collection.

Type of specimen: Suspected patient: At least 2 ml of fresh blood in sodium heparin tube (green-top) AND healthy control (at least 2 ml of fresh blood in sodium heparin tube)

Method/Principle: Flow cytometry (CFSE)

Interpretation: Percentage of T cells that proliferate in the suspected patient compared with healthy control samples. 

Clinical significance: Severe combined immunodeficiency (SCID) and other primary immunodeficiency disorders. 

Results: Within 7 working days.

Note: An appointment with the Laboratory is essential for this test (at least one day earlier). All specimen must arrive at Immunology Laboratory before 10am and within 24 hours of collection.